重组人 TNFR2 / CD120b / TNFRSF1B 蛋白 (ECD, His标签)（产品说明）
蛋白名称：Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Asp257) of human TNFRII (NP_001057.1) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α. 2. Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha (Cat:10602-HNAE) at 2 μg/ml (100 μl/well) can bind Human TNFR2/TNFRSF1B His(Cat:10417-H08H), the EC50 of Human TNFR2/TNFRSF1B His is 2.0-12.0 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 23
蛋白分子量：The recombinant human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 40.1 kDa due to glycosylation.
蛋白NP号：NP_001057.1
蛋白氨基酸序列：Met1-Asp257
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.