重组人 TNFR2 / CD120b / TNFRSF1B 蛋白 (ECD, His & Fc标签)（产品说明）
蛋白名称：Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His & Fc Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Asp257) of human TNFRII (NP_001057.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-16 ng/ml in the presence of 0.25 ng/mL recombinant human TNF-α.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 23
蛋白分子量：The recombinant human TNFRII/Fc is a disulfide-linked homodimer. The reduced monomer consists of 482 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII/Fc monomer is approximately 65.6 kDa due to glycosylation.
蛋白NP号：NP_001057.1
蛋白氨基酸序列：Met1-Asp257
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.