图文详情
小鼠 TNFRSF1A蛋白|TNFRSF1A protein|TNFRSF1A(Mouse, C-His)(产品说明)
蛋白名称:TNFRSF1A蛋白, TNFRSF1A protein
蛋白构建:A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL. 3. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 30
蛋白分子量:The secreted recombinant mouse TNFRSF1A consists of 194 amino acids and has a predicted molecular mass of 21.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A is approximately 35 kDa due to glycosylation.
蛋白NP号:NP_035739.2
蛋白氨基酸序列:Met1-Ala212
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.