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收藏TIGIT重组蛋白|Recombinant Human TIGIT Protein (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Human TIGIT Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human TIGIT (NP_776160.2) (Met22-Pro141) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human TIGIT Protein (His & AVI Tag), Biotinylated (Cat: 10917-H49H1-B)at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human CD155 / PVR / NECL5 Protein (Fc Tag) (Cat: 10109-H02H), the EC50 is 15-45 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met 22
蛋白分子量:The recombinant Human TIGIT consists of 286 amino acids and predicts a molecular mass of 30.63 kDa. It migrates as an approximately 39.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_776160.2
蛋白氨基酸序列:Met22-Pro141
蛋白标签:C-His-AVI