TIGIT重组蛋白|Recombinant Human TIGIT Protein (ECD, Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human TIGIT Protein (ECD, Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human TIGIT (NP_776160.2) (Met1-Pro141) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human CD155/PVR His (Cat: 10109-H08H) at 2 μg/mL (100 μl/well) can bind Human TIGIT (hFc & AVI Tag), Biotinylated (Cat: 10917-H41H-B)，The EC50 of bind Human TIGIT (hFc & AVI Tag), Biotinylated is 10-60 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 22
蛋白分子量：The recombinant human TIGIT consists of 373 amino acids and predicts a molecular mass of 41.6 kDa. As a result of glycosylation, it migrates as an approximately 49.2 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_776160.2
蛋白氨基酸序列：Met1-Pro141
蛋白标签：C-human IgG1-Fc & AVI