TFPI重组蛋白|Recombinant Mouse TFPI / LACI / EPI Protein (His Tag)（产品说明）
蛋白名称：Mouse TFPI / LACI / EPI Protein (His Tag)
蛋白构建：A DNA sequence encoding the mouse TFPI isoform alpha (NP_035706.1) (Met 1-Lys 289) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog # 27114). The IC50 value is < 0.5 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog # T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2 , 0.15 M NaCl, 0.05% Brij 35, pH 7.5 .
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 29
蛋白分子量：The secreted recombinant mouse TFPI consists of 272 amino acids and has a calculated molecular mass of 31 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035706.1
蛋白氨基酸序列：Met1-Lys289
蛋白标签：C-His