重组新冠病毒 JN.1 棘突 S1+S2 trimer 蛋白 (His & AVI 标签)（产品说明）
蛋白名称：SARS-COV-2 JN.1 (Omicron) Spike S1+S2 trimer Protein (His & AVI Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (JN.1) Spike S1+S2 trimer (YP_009724390.1, with mutations ins16MPLF, T19I, R21T, L24del, P25del, P26del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, R682G, R683S, R685S, N764K, D796Y, F817P, A892P, A899P, S939F, A942P, Q954H, N969K, K986P, V987P, P1143L and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant JN.1).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性：Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 JN.1 Spike S1+S2 trimer (His & AVI Tag), Biotinylated (Cat: 40589-V49H15-B), the EC50 is 3-10 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (JN.1) Spike S1+S2 trimer consists of 1242 amino acids and predicts a molecular mass of 138.32 kDa. The molecular weight of this protein is around 524.6 kDa verified by SEC-MALS.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208