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重组新冠病毒Coronavirus spike蛋白(产品说明)
蛋白名称:SARS-CoV-2 NB.1.8.1 (Omicron) Spike RBD Protein (His Tag)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (NB.1.8.1) Spike RBD (YP_009724390.1, with mutation I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, A435S, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478I, N481K, del483, E484K, F486P, Q493E, Q498R, N501Y, Y505H) (Arg319-Lys529) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant NB.1.8.1).
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE.Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 NB.1.8.1 (Omicron) Spike RBD Protein (His Tag) (Cat: 40592-V08H161), the EC50 is 15-50 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Arg 319
蛋白分子量:The recombinant SARS-CoV-2 Spike RBD consists of 221 amino acids and predicts a molecular mass of 25.3 kDa. As a result of glycosylation, it migrates as an approximately 36.6 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Lys529