重组新冠病毒Coronavirus spike蛋白（产品说明）
蛋白名称：SARS-COV-2 BA.1 (Omicron) Spike S1+S2 trimer Protein (His & AVI Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.1) Spike S1+S2 trimer (YP_009724390.1, with mutations A67V, del69-70, T95I, G142D, del143-145, N211I, del212, G339D, R346K, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, R682G, R683S, R685S, N764K, D796Y, F817P, N856K, A892P, A899P, A942P, Q954H, N969K, L981F, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.1).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (BA.1) Spike S1+S2 trimer consists of 1240 amino acids and predicts a molecular mass of 138.07 kDa. It migrates as an approximately 182.6 and 147.9 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 547.9 kDa verified by SEC-MALS.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208