重组新冠病毒Coronavirus spike蛋白（产品说明）
蛋白名称：SARS-COV-2 LF.7 (Omicron) Spike S1+S2 trimer Protein (His & AVI Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (LF.7) Spike S1+S2 trimer (YP_009724390.1, with mutations ins16MPLF, T19I, R21T, T22N, L24S, del25/27, S31P, S50L, del69/70, V127F, G142D, del144/144, F157S, R158G, K182R, R190S, N211I, del212/212, V213G, L216F, H245N, A264D, I332V, G339H, R346T, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, K444R, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, del483/483, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, R682G, R683S, R685S, N764K, D796Y, F817P, A892P, A899P, S939F, A942P, Q954H, N969K, K986P, V987P, P1143L and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant LF.7).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 LF.7 Spike S1+S2 trimer (His & AVI Tag), Biotinylated (Cat: 40589-V49H13-B), the EC50 is 40-120 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (LF.7) Spike S1+S2 trimer consists of 1242 amino acids and predicts a molecular mass of 138.24 kDa. It migrates as an approximately 187.9 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 544.3 kDa verified by SEC-MALS.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208