Coronavirus spike重组蛋白|Recombinant Coronavirus JN.1 Spike RBD Protein(aa319-529,His&AVI Tag),Biotinylated（产品说明）
蛋白名称：Coronavirus JN.1 (Omicron) Spike RBD Protein (aa319-529, His & AVI Tag), Biotinylated(HPLC-verified)
蛋白构建：A DNA sequence encoding the Coronavirus Spike RBD (YP_009724390.1, with mutation I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H) (Arg319-Lys529) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the Coronavirus variant (known as variant JN.1).
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant Coronavirus JN.1 Spike RBD Protein(aa319-529,His&AVI Tag), Biotinylated (Cat: 40592-V49H16-B), the EC50 is 3-9 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Arg 319
蛋白分子量：The recombinant Coronavirus (JN.1) Spike RBD consists of 236 amino acids and predicts a molecular mass of 27.15 kDa. As a result of glycosylation, it migrates as an approximately 38.5 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Lys529
蛋白标签：C-His-AVI