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Coronavirus spike重组蛋白|Recombinant Coronavirus EG.5.1 Spike RBD Protein(aa319-529,His&AVI Tag),Biotinylated(产品说明)
蛋白名称:Coronavirus EG.5.1 (Omicron) Spike RBD Protein (aa319-529, His & AVI Tag), Biotinylated(HPLC-verified)
蛋白构建:A DNA sequence encoding the Coronavirus Spike RBD (YP_009724390.1, with mutation G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H) (Arg319-lys529) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the Coronavirus variant (known as variant EG.5.1).
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant Coronavirus EG.5.1 Spike RBD Protein(aa319-529,His&AVI Tag),Biotinylated (Cat: 40592-V49H15-B), the EC50 is 7-21 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Arg 319
蛋白分子量:The recombinant Coronavirus Spike RBD consists of 237 amino acids and predicts a molecular mass of 27.01 kDa. As a result of glycosylation, it migrates as an approximately 35.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-lys529
蛋白标签:C-His-AVI