重组新冠病毒 (BA.2.12.1) Spike S1+S2 trimer 蛋白(ECD,His&AVI 标签)（产品说明）
蛋白名称：SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein (ECD, His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, L24S, del25-27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.12.1).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein(ECD,His&AVI Tag) (Cat: 40589-V49H7-B), the EC50 is 3-10 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer consists of 1245 amino acids and predicts a molecular mass of 138.61 kDa.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208
蛋白标签：C-His-AVI
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.