重组新冠病毒(BA.4/BA.5) S1+S2 trimer 蛋白 (ECD, His & AVI 标签)（产品说明）
蛋白名称：SARS-CoV-2 (BA.4/BA.5) Spike S1+S2 trimer Protein (ECD, His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.4/BA.5) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, L24del, P25del, P26del, A27S, H69del, V70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) (Met1-1208Q) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4/BA.5).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well)  can bind Recombinant SARS-CoV-2(BA.4/BA.5) S1+S2 trimer Protein (ECD, His & AVI Tag) (Cat:40589-V49H5-B), the EC50 is 2.7-8.1 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 16
蛋白分子量：The recombinant SARS-CoV-2 (BA.4/BA.5) Spike S1+S2 trimer consists of 1241 amino acids and predicts a molecular mass of 138.07 kDa.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Met1-Gln1208
蛋白标签：C-His-AVI
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.