重组新冠病毒 (BA.2.75) Spike RBD 蛋白 (His 标签)（产品说明）
蛋白名称：SARS-CoV-2 (BA.2.75) Spike RBD Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.75) Spike RBD (YP_009724390.1, with mutations G339H,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,G446S,N460K,S477N,T478K,E484A,Q498R,N501Y,Y505H)(Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.75).
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.75) Spike RBD Protein (His Tag)(Cat:40592-V08H135), the EC50 is 3-10ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (BA.2.75) Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.75 kDa. As a result of glycosylation, it migrates as an approximately 38.11 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.