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重组新冠病毒 (BA.2.12.1) Spike S1+S2 trimer 蛋白 (ECD, His标签)(产品说明)
蛋白名称:SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (BA.5) Spike S1+S2 (YP_009724390.1, with mutations T19I, L24S, del25-27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.5).
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Human ACE2 (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 trimer Protein (ECD, His Tag)(Cat:40589-V08H34), the EC50 is 5-20 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 16
蛋白分子量:The recombinant SARS-CoV-2 (BA.2.12.1) Spike S1+S2 consists of 1228 amino acids and predicts a molecular mass of 136.57 kDa.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:0
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.