重组新冠病毒 (BA.2.9.1 and BA.2.13) Spike RBD 蛋白 (His标签)（产品说明）
蛋白名称：SARS-CoV-2 (BA.2.9.1 and BA.2.13) Spike RBD Protein (His Tag)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.9.1 and BA.2.13) Spike RBD (YP_009724390.1, with mutations G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452M, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)(Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.9.1 and BA.2.13).
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.9.1 and BA.2.13) Spike RBD Protein (His Tag)(Cat:40592-V08H134), the EC50 is 10-30ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (BA.2.9.1 and BA.2.13) Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.72 kDa. As a result of glycosylation, it migrates as an approximately 35.43 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.