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Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 (BA.1.1) Spike RBD Protein (His Tag)(产品说明)
蛋白名称:SARS-CoV-2 (BA.1.1) Spike RBD Protein (His Tag) (HPLC-verified)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (BA.1.1) Spike RBD (YP_009724390.1, with mutations G339D, R346K, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)(Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.1.1).
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.1.1) Spike RBD Protein (His Tag) (Cat:40592-V08H129), the EC50 is 3-10 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Arg 319
蛋白分子量:The recombinant SARS-CoV-2 (BA.1.1) Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.78 kDa. As a result of glycosylation, it migrates as an approximately 33.95 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Phe541