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重组新冠病毒 (BA.1.1) Spike S1+S2 trimer 蛋白 (ECD, His标签)(产品说明)
蛋白名称:SARS-CoV-2 (BA.1.1) Spike S1+S2 trimer Protein (ECD, His Tag)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (BA.1.1) Spike S1+S2 trimer (YP_009724390.1, with mutations A67V, del69-70, T95I, G142D, del143-145, N211I, del212, G339D, R346K, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, N856K, A892P, A899P, A942P, Q954H, N969K, L981F, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.1.1).
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (AY.4.2) Spike S1+S2 trimer Protein ( ECD, His Tag)(Cat:40589-V08H29), the EC50 is 4-18 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 16
蛋白分子量:The recombinant SARS-CoV-2 (BA.1.1) Spike S1+S2 trimer consists of 1225 amino acids and predicts a molecular mass of 136.26 kDa.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Met1-Gln1208
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.