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Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 Spike S1(K417N,E484K,N501Y,D614G)-His Protein(产品说明)
蛋白名称:SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein (YP_009724390.1) (Met1-Arg685(K417N, E484K, N501Y, D614G)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His(Cat:40591-V08H10), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His(Cat:40591-V08H10) is 15-90 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 16
蛋白分子量:The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.4 kDa. As a result of glycosylation, it migrates as an approximately 114.7 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Met1-Arg685(K417N, E484K, N501Y, D614G)