新冠病毒SARS-CoV-2 Spike RBD(N501Y)-His 重组蛋白（产品说明）
蛋白名称：新冠病毒N501Y突变体蛋白
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N501Y)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(40592-V08H82), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His (40592-V08H82) is 3-20 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541(N501Y)
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.