重组人CD98蛋白(AF 647定点偶联, His标签)（产品说明）
蛋白名称：Human CD98 Protein (Site-Specific AF 647 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human SLC3A2 isoform 1 (P08195-1) (Arg 206-Ala 630) was expressed with a N-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-CD98 CAR expression. 5e5 of anti-CD98 CAR-293 cells were stained with 100 μL of 3 μg/mL of AF 647-conjugated human CD98 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 12206-H85H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated CD98 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human CD98 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 12206-H85H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.Gly
蛋白分子量：The protein has a predicted molecular mass of 50.5 kDa.
蛋白NP号：P08195-1
蛋白氨基酸序列：Arg206-Ala630