重组人 CD31 / PECAM1 蛋白 (His标签)（产品说明）
蛋白名称：Human CD31 / PECAM1 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human CD31 (EAW94208.1) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31-His coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA,approximately >70% will adhere after 30 minutes at 37℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 28
蛋白分子量：The secreted recombinant human CD31 consists of 585 amino acids after removal and predicts a molecular mass of 66 kDa. As a result of glycosylation, the rhCD31 migrates as approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：EAW94208.1
蛋白氨基酸序列：Met1-Lys601
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.