图文详情
重组人 CD31 / PECAM1 蛋白 (Fc标签)(产品说明)
蛋白名称:Human CD31 / PECAM1 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human CD31 (EAW94208.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10E4 cells/well are added to sCD31-Fc coated plates (5 μg/mL and 100 μL/well) in the presence of 10 μg/ml PHA approximately >60% will adhere after 30 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 28
蛋白分子量:The recombinant human CD31/Fc is a disulfide-linked homodimer.The reduced monomer consists of 812 amino acids and predicts a molecular mass of 91.2 kDa. As a result of glycosylation, the rhCD31/Fc monomer migrates as approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:EAW94208.1
蛋白氨基酸序列:Met1-Lys601
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.