图文详情
重组人PD-1蛋白 (His & AVI 标签)(产品说明)
蛋白名称:Human PD-1 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human PDCD1 (NP_005009.2)(Met1-Gln167) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human PD-1 Protein (His & AVI Tag), Biotinylated (Cat: 10377-H49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10084-H02H), the EC50 is 1-4 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Leu 25
蛋白分子量:The recombinant Human PDCD1 comprises 169 amino acids and has a predicted molecular mass of 19.21 kDa. It migrates as approximately 42.9 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_005009.2
蛋白氨基酸序列:Met1-Gln167