图文详情
重组人 NECTIN4/Nectin 4 蛋白 (ECD, Fc & AVI 标签), 生物素标记(产品说明)
蛋白名称:Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 19771-H41H-B)at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL,100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag) (Cat: 11611-H08H,the EC50 is 40-120 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gly 32
蛋白分子量:The recombinant Human NECTIN4 consists of 571 amino acids and predicts a molecular mass of 62.65 kDa. It migrates as an approximately 76.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_112178.2
蛋白氨基酸序列:Met1-Ser349
蛋白标签:C-human IgG1-Fc & AVI
蛋白保存条件:-20℃ to -80℃