重组人 CD111 / Nectin-1 / PVRL1 蛋白 (His标签)（产品说明）
蛋白名称：Human CD111 / Nectin-1 / PVRL1 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml. 2. Immobilized Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10852-H02H) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag)(Cat:11611-H08H), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag)is 150-700 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 31
蛋白分子量：The recombinant human PVRL1 consists of 315 amino acids and predictes a molecular mass of 35.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPVRL1 is approximately 45-50 kDa due to glycosylation.
蛋白NP号：NP_002846.3
蛋白氨基酸序列：Met1-Thr334
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.