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收藏小鼠 c-MET蛋白|c-MET protein|c-MET(Mouse, C-human IgG1-Fc)(产品说明)
蛋白名称:c-MET蛋白, c-MET protein
蛋白构建:A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human HGF at 2 μg/ml (100 μl/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 25 & Ser 307
蛋白分子量:The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1146 amino acids and has a predicted molecular mass of 128 (α =32 + Fc tagged β=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:NP_032617.2
蛋白氨基酸序列:Met1-Asn929
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.