图文详情
重组人 c-MET / HGFR 蛋白 (His标签)(产品说明)
蛋白名称:Human c-MET / HGFR Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain of human c-Met (NP_000236.2) (Met1-Thr932) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Immobilized Cynomolgus HGF(Cat:90286-CNAH) at 2 μg/mL (100 μL/well) can bind Human c-MET His(Cat:10692-H08H), the EC50 of Human c-MET His(Cat:10692-H08H) is 20-120ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 25
蛋白分子量:The mature form of recombinant human c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 919 amino acids and has a predicted molecular mass of 103 (α=33 +β=70) kDa. As a result of glycosylation, rh c-MET heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 85 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:NP_000236.2
蛋白氨基酸序列:Met1-Thr932
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.