LRP-6重组蛋白|Recombinant Human LRP6 Protein (His & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human LRP6 Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human LRP6 (O75581) (Met1-Pro630) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：1.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, His Tag (Cat. No. 10170-H08H) with an affinity constant of 90.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human R-Spondin 1/RSPO1 Protein, His Tag (Cat. No. 11083-H08H) with an affinity constant of 0.983 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested). 3.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human SOST/Sclerostin Protein, His Tag (Cat. No. 10593-H07H) with an affinity constant of 39.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, hFc Tag (Cat. No. 10170-H02H) with an affinity constant of 54.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ala 20
蛋白分子量：The recombinant Human LRP6 consists of 637 amino acids and predicts a molecular mass of 72.22 kDa. It migrates as an approximately 79.8 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：O75581
蛋白氨基酸序列：Met1-Pro630
蛋白标签：C-His-AVI