图文详情
重组人 Leptin Receptor / LEPR / CD295 蛋白 (His & Fc标签)(产品说明)
蛋白名称:Human Leptin Receptor / LEPR / CD295 Protein (His & Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human leptin receptor precursor (NP_002294.2) was expressed with the C-terminal fused polyhistidine-tagged Fc region of human IgG1.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/ml .
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 22
蛋白分子量:The recombinant human LEPR/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 1065 amino acids and has a predicted molecular mass of 121.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR/Fc monomer is approximately 155-165 kDa due to glycosylation.
蛋白NP号:NP_002294.2
蛋白氨基酸序列:Met1-Asp839
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.