图文详情
人 LDLR/LDL Receptor蛋白|LDLR/LDL Receptor protein|LDLR/LDL Receptor(Human, C-His)(产品说明)
蛋白名称:LDLR/LDL Receptor蛋白, LDLR/LDL Receptor protein
蛋白构建:A DNA sequence encoding the extracellular domain of human LDLR (NP_000518.1) precursor (Met1-Arg788) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measure by its ability to bind with human PCSK9 in a functional ELISA. 1. Immobilized human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.61 μg/ml. 2. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 22
蛋白分子量:The recombinant human LDLR consists of 777 amino acids and predicts a molecular mass of 86 kDa.
蛋白NP号:NP_000518.1
蛋白氨基酸序列:Met1-Arg788
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.