重组小鼠c-Kit蛋白(AF 488定点偶联,胞外区)（产品说明）
蛋白名称：Human c-Kit Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human Kit (P10721-2) (Met 1-Thr 516) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Flow cytometric analysis of anti-c-Kit CAR expression. 5e5 of anti-c-Kit CAR-293 cells were stained with 100 μL of 6μg/ml of AF 488-conjugated human c-Kit Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 11996-H86H-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated c-Kit protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human c-Kit Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 11996-H86H-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 26
蛋白分子量：The protein has a predicted molecular mass of 58.6 kDa.
蛋白NP号：P10721-2
蛋白氨基酸序列：Met 1-Thr 516