重组人 IL6R / CD126 蛋白 (His标签)（产品说明）
蛋白名称：Human IL6R / CD126 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met1-Pro365) was expressed with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/mL (100μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/ml. 2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 10-60 ng/mL. 3. Using the Octet RED System, the affinity constant (Kd) of IL-6R Protein, Human, Recombinant (His Tag) (Cat. 10398-H08H) bound Anti-IL6R Antibody (Cat. 10398-MM08) was 0.1 nM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Leu 20
蛋白分子量：The soluble form of recombinant human IL6R consists of 357 amino acids and has a predicted molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 60-65 kDa due to glycosylation.
蛋白NP号：NP_000556.1
蛋白氨基酸序列：Met1-Pro365
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.