图文详情
IL-5重组蛋白|Recombinant Human IL-5 Protein (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Human IL-5 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human IL5 (NP_000870.1) (Ile20-Ser134) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性:Immobilized Recombinant Human IL-5 Protein (His & AVI Tag), Biotinylated (Cat: 15673-H49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL-5R alpha Protein (Membrane-bound, Fc Tag) (Cat: 10392-H01H1), the EC50 is 20-60 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ile 20
蛋白分子量:The recombinant Human IL5 comprises 141 amino acids and has a predicted molecular mass of 16.41 kDa. It migrates as approximately 21 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 39.3 kDa verified by SEC-MALS.
蛋白NP号:NP_000870.1
蛋白氨基酸序列:Ile20-Ser134