小鼠 IL-4蛋白|IL-4 protein|IL-4(Mouse, C-His)（产品说明）
蛋白名称：IL-4蛋白, IL-4 protein
蛋白构建：A DNA sequence encoding the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His (Cat:51084-M08B) at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc (Cat:80198-R02H), The EC50 of rat IL4R-Fc (Cat:80198-R02H) is 0.12-0.29 μg/mL. 2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：His 21
蛋白分子量：The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P07750
蛋白氨基酸序列：Met 1-Ser140, Q136D, Y139D
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.