图文详情
Recombinant Huamn IL-31RA 蛋白 (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Huamn IL-31RA Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Huamn IL-31RA (EAW54932.1) (Met1-Thr516) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Huamn IL-31RA Protein (His & AVI Tag), Biotinylated (Cat: 15878-H49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-IL31RA(Research Grade Nemoliz*mab Biosimilar), the EC50 is 2-7 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 20
蛋白分子量:The recombinant Huamn IL-31RA comprises 510 amino acids and predicts a molecular mass of 58.6 kDa. As a result of glycosylation, it migrates as an approximately 105.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:EAW54932.1
蛋白氨基酸序列:Met1-Thr516