图文详情
重组人 IL-31 蛋白 (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Human IL-31 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human IL-31 (NP_001014358.1) (Ser24-Thr164) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性:Immobilized Recombinant Human IL-31 Protein (His & AVI Tag), Biotinylated (Cat: 11557-H56H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human Interleukin-31 receptor A / IL31RA Protein (Fc Tag, ECD) (Cat: 15878-H02H) , the EC50 is 100-300 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:His
蛋白分子量:The recombinant Human IL-31 comprises 184 amino acids and has a predicted molecular mass of 20.23 kDa. It migrates as an approximately 34.7 and 28.3 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 30.4 kDa verified by SEC-MALS.
蛋白NP号:NP_001014358.1
蛋白氨基酸序列:Ser24-Thr164