CD25/IL-2R alpha重组蛋白|Recombinant Human CD25/IL-2R alpha Protein (Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human CD25/IL-2R alpha Protein (Fc & AVI Tag), BiotinylatedA DNA sequence encoding the Human IL2RA (NP_000408.1) (Met1-Cys213) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).Immobilized Recombinant Human CD25/IL-2R alpha Protein (Fc & AVI Tag), Biotinylated (Cat: 10165-H41H-BB) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL2 Protein (His Tag) (Cat: 11848-H08H), the EC50 is 0.5-2.5 ng/mL (QC tested).< 1.0 EU per μg protein as determined by the LAL method.Glu 22The recombinant Human IL2RA comprises 445 amino acids and has a predicted molecular mass of 50.28 kDa. It migrates as approximately 65.9 kDa and 37.5 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 117.2 kDa verified by SEC-MALS(Routinely tested).NP_000408.1Met1-Cys213