重组人 IL27 / Interleukin-27 蛋白 (His标签)（产品说明）
蛋白名称：Human IL27 / Interleukin-27 Protein (His Tag)
蛋白构建：A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.
表达宿主：CHO Stable Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.2-0.46 μg/ml. 2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Arg 21
蛋白分子量：The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_005746.2 & NP_663634.2
蛋白氨基酸序列：Met1-Lys229 & Phe29-Pro243
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.