图文详情
小鼠 IL25蛋白|IL25 protein|IL25(Mouse, N-His)(产品说明)
蛋白名称:IL25蛋白, IL25 protein
蛋白构建:A DNA sequence encoding the mature form of mouse IL25 (NP_542767.1) (Val 17-Ala 169) was expressed with a N-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 80 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL25-His (Cat:50138-M07H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse IL17BR-His (Cat:51109-M08H) with a linear range of 3.125-50 ng/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 4-14ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gly
蛋白分子量:The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_542767.1
蛋白氨基酸序列:Val 17-Ala 169
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.