重组人 IL25 蛋白 (Fc标签)（产品说明）
蛋白名称：Human IL25 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the mature form of human IL25 (Tyr 33-Gly177) (NP_073626.1) was fused with the Fc region of human IgG1 at the N-terminus via a polypeptide linker.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His (Cat:13091-H08H) at 10 μg/ml (100 μl/well) can bind human Fc-IL25 (Cat:10096-H01H), The EC50 of human Fc-IL25 (Cat:10096-H01H) is 1.7-4.2 μg/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 20
蛋白分子量：The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_073626.1
蛋白氨基酸序列：Tyr 33-Gly177
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.