图文详情
小鼠 IL-1 alpha/IL1A蛋白|IL-1 alpha/IL1A protein|IL-1 alpha/IL1A(Mouse, Native)(产品说明)
蛋白名称:IL-1 alpha/IL1A蛋白, IL-1 alpha/IL1A protein
蛋白构建:A DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270) was expressed, with an initial Met at the C-terminus.
表达宿主:E. coli
蛋白纯度:> 92 % as determined by SDS-PAGE
蛋白活性:1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-10 pg/mL. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL. 4. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 3-15 ng/mL.
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号:NP_034684.2
蛋白氨基酸序列:Ser115-Ser270
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.