IL-23重组蛋白|Recombinant Human IL23A & Mouse IL12B Heterodimer Protein (His Tag)（产品说明）
蛋白名称：Human IL23A & Mouse IL12B Heterodimer Protein (His Tag)
蛋白构建：A DNA sequence encoding the Human IL23A (P43432) (Met23-Ser335) was expressed, constructed the plasmid 1; A DNA sequence encoding the Mouse IL12B (NP_057668.1) (Met1-Pro189) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL23A & Mouse IL12B Heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性：1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL can bind Recombinant Human IL23A & Mouse IL12B Heterodimer Protein (His Tag) (Cat: CT155-A08H), the EC50 is 5-15 ng/mL(QC tested). 2.Immobilized Recombinant Human IL23A & Mouse IL12B Heterodimer Protein (His Tag) (Cat: CT155-A08H) at 2 μg/mL can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 80-250 ng/mL (Routinely tested).
蛋白内毒素：< 50 EU per mg protein.
预测N端：Met 23 & Arg 20
蛋白分子量：The recombinant heterodimer of Human IL23A & Mouse IL12B comprises 494 (313+181) amino acids and has a calculated molecular mass of 55.93 (35.8+20.13) kDa. As a result of glycosylation, the apparent molecular mass of Human IL23A & Mouse IL12B Heterodimer is approximately 45.5 kDa、42.2 kDa and 21.7 kDa in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 61.4 kDa verified by SEC-MALS.
蛋白NP号：P43432 & NP_057668.1
蛋白氨基酸序列：Met23-Ser335 & Met1-Pro189