图文详情
重组大鼠 IL-23 (IL23A & IL12B 异源二聚体) 蛋白(产品说明)
蛋白名称:Rat IL-23 (IL23A & IL12B Heterodimer) Protein
蛋白构建:A DNA sequence encoding the rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-3ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 22 & Met 23
蛋白分子量:The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
蛋白NP号:NP_569094.1 & EDM04146.1
蛋白氨基酸序列:Met1-Ala196 & Met1-Ser335
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.