图文详情
小鼠 IL12B蛋白|IL12B protein|IL12B(Mouse, C-human IgG1-Fc)(产品说明)
蛋白名称:IL12B蛋白, IL12B protein
蛋白构建:A DNA sequence encoding the mouse IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse IL12B-Fc at 10μg/mL (100μL/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H). The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 0.10-0.24μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met 23
蛋白分子量:The secreted recombinant mouse IL12B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 554 amino acids and has a calculated molecular mass of 62.8 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 83 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P43432
蛋白氨基酸序列:Met1-Ser335
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.