重组人 IL-12 (IL12A & IL12B 异源二聚体) 蛋白 (His标签)（产品说明）
蛋白名称：Human IL-12 (IL12A & IL12B Heterodimer) Protein (His Tag)
蛋白构建：A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL.
蛋白内毒素：< 5 EU per mg of the protein.
预测N端：Arg 23 & Ile 23
蛋白分子量：The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208+317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.
蛋白NP号：P29459-1 & NP_002178.2
蛋白氨基酸序列：Met1-Ser219 & Met1-Ser328
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.