图文详情
重组恒河猴 IL10RA 蛋白 (Fc标签)(产品说明)
蛋白名称:Rhesus IL10RA Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the rhesus IL10RA (XP_001092736.1) (Thr9-Asn245) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IL10RA-Fc (cat:90125-C02H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL10 (Cat:10947-H07H), The EC50 of biotinylated human IL10 (Cat:10947-H07H) is 39.0-92.0 ng/ml. 2. Measured by its ability to inhibit IL-10 dependent proliferation of MC/9 mouse mast cells. Approximately 0.1-0.4 μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 29
蛋白分子量:The recombinant rhesus IL10RA comprises 458 amino acids and has a calculated molecular mass of 51.8 KDa.
蛋白NP号:XP_001092736.1
蛋白氨基酸序列:Thr9-Asn245
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.