IGFBP7 Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated, HPLC-verified: 产品信息
纯度≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物素标记率0.5-1 as determined by the HABA assay.
生物活性Immobilized Recombinant Human CD93 / C1QR1 Protein (His Tag) ( Cat:12589-H08H) at 2μg/mL (100μL/well) can bind Recombinant Human IGFBP7 Protein (Fc & AVI Tag), Biotinylated (Cat: 13100-H41H-B), the EC50 is 7-21 ng/mL.
蛋白构建A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu282) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主HEK293 Cells
种属Human
预测 N 端Ser 27
分子量The recombinant human IGFBP7 consists of 509 amino acids and predicts a molecular mass of 54.98 kDa. It migrates as an approximately 62.49 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

IGFBP7 Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated, HPLC-verified: 图片




IGFBP7 Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated, HPLC-verified: 别称
AGM Protein, Human; FSTL2 Protein, Human; IBP-7 Protein, Human; IGFBP-7 Protein, Human; IGFBP-7v Protein, Human; IGFBPRP1 Protein, Human; MAC25 Protein, Human; PSF Protein, Human; RAMSVPS Protein, Human; TAF Protein, Human
IGFBP7 背景信息
Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.
全称
insulin-like growth factor binding protein 7