图文详情
重组人 IGFBP7 / IBP-7 蛋白 (Fc标签)(产品说明)
蛋白名称:Human IGFBP7 / IBP-7 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the human IGFBP7 (Q16270) (Met 1-Leu 282) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . 1. Immobilized IGFBP7-Fc at 20 μg/ml (100 μl/well) can bind biotinylated human IGF2-nusa. The EC50 of biotinylated human IGF2-nusa is 0.06 μg/ml . 2. Immobilized human CCL21 at 2 μg/ml (100 μl/well) can bind human IGFBP7-Fc with a linear range of 0.16-4 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 27
蛋白分子量:The secreted recombinant human IGFBP7/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 53.5 kDa. The apparent molecular mass of rh IGFBP7/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q16270
蛋白氨基酸序列:Met1-Leu282
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.